Research area
- We aim to understand the principles of cell chemistry under the lens of structural biology.
- Currently, we are interested in the coevolutionary arms race between bacteria and bacteriophages,
focusing on mechanistic investigations of anti-CRISPR proteins.
- Structural mechanism of CRISPR-Cas and anti-CRISPR functions
- Bacteria and archaea employ the CRISPR-Cas system to destroy nucleic acids of phages and plasmids upon infection.
- Phages developed CRISPR inhibitor (anti-CRISPR) proteins to neutralize the bacterial defense system.
- We try to understand the mode of action and molecular mechanisms underlying the CRISPR inhibition.
- References
Nucleic Acids Res. (2022) 50, 2363-2376
CRISPR J. (2021) 4, 448-458
Nucleic Acids Res. (2020) 48, 9959-9968
Nucleic Acids Res. (2020) 48, 7584-7594
FEBS J. (2019) 286, 4661-4674
Sci. Rep. (2018) 8, 3883
Nucleic Acids Res. (2018) 46, 485-492
J. Biol. Chem. (2018) 293, 2744-2754
FEBS Lett. (2018) 286, 4661-4674
- Detection of lowly populated transient species using NMR paramagnetic relaxation to probe protein interactions and motions
- NMR paramagnetic relaxation enhancement (PRE) is highly sensitive and useful to detect short-lived transient species.
- We employ the PRE to monitor metastable states, such as target search process during protein association or dynamic equilibrium of a conformational ensemble.
- References
Structure (2018) 26, 887-893
Angew. Chem. Int. Ed. Engl. (2013) 52, 3384-3388
- Protein-ligand interaction and Structure-Activity Relationship(SAR) by NMR
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